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External Education Websites on Hearing

Journey into the World of Hearing

by Rémy Pujol et al., INSERM and University of Montpellier

This is an excellent educational website, with a section for Students and Professionals at  www.cochlea.eu/en and a section for the public at www.cochlea.org/en.

 


ARO 2020 Photo Contest

Below you will find stunning images from the research labs of our members, submitted in response to an ARO Art Contest held in January 2020.  These images can be seen scattered throughout our web pages.  Please make sure that you do not use these images without crediting the source.  Thank you, and enjoy!

First Place

Credit:  Patrick Lam, Ella Trang, Niki Gunewardene, and Andrew Wise of Bionics Institute

Description: 3-day culture of primary spiral ganglion cells isolated from 5D neonatal Sprague Dawley rats.This image shows a common phenomenon of spiral ganglion neuron processes being surrounded by glial cells that could play an important role in the nerve survival and signalling.Cells were visualised via immunocytochemistry. Labelled in green are two spiral ganglion neurons using Tuj1 as the primary antibody binding marker; in red are the glial cells using S100 beta as the primary antibody binding marker; all cell nuclei were stained with DAPI in blue.


Second Place- Tied

Credit: David Bächinger, Andreas H. Eckhard

Description: This image shows a quadruple immunostaining a of a whole-mount specimen of the adult murine endolymphatic sac (6 weeks old female C57BL/6J). Lymphatic vessels were stained with an anti-LYVE1 antibody (yellow). Concanavalin A (green) was used to mark blood vessels. The endolymphatic sac is outlined by epithelial staining using a pan-cytokeratin antibody (red). Cell nuclei are marked with DAPI (blue). Note that lymphatic (yellow) and blood vessels (red) form a “star-like” hub in the endolymphatic sac region


Second Place- Tied

Credit: Leonardo R. Andrade of Salk Institute for Biological Studies

Description: SEM image of bullfrog otoconia.


 

Honorable Mentions – 18 photos in no specific order.


Credit: David Bächinger, Andreas H. Eckhard

Description: This image shows an overview of an immunostained section of the adult murine endolymphatic sac (6 weeks old female C57BL/6J).The endolymphatic sacepithelium is outlined using acytokeratin 7 antibody(red) and a calcium sensing receptor (CaSR) antibody(green).Cell nuclei are marked with DAPI (blue).Note that CaSR staining is found exclusively in a subset of cells, which were shown the mitochondria-rich cells of the endolymphatic sac (Bächinger Det al.,Cell Tissue Res.2019). An excerpt of this image appeared on the cover page of the journal “Cell and Tissue Research” (Volume 378, November 2019


Credit: Image is submitted on behalf of freelance Malaysian born artist, Mr Jeganathan Ramachandran by Deborah Hall, Professor of Hearing Sciences, University of Nottingham Malaysia

Description: Artist’s impression of auditory processing in human auditory cortex as captured in two-dimensional form using functional Magnetic Resonance Imaging. Ink drawing on paper, 2018


Credit: Image is submitted on behalf of freelance Malaysian born artist, Mr Jeganathan Ramachandran by Deborah Hall, Professor of Hearing Sciences, University of Nottingham Malaysia

Description: Artist’s impression of the transfer of acoustic energy into neural signals in the human inner ear. Ink drawing on paper, 2018


Credit: Hainan Lang MD, PhD; Medical University of South Carolina; Department of Pathology and Laboratory Medici

Description: A section of the auditory nerve in a young adult CBA/CaJ mouse showing how glial cell associated myelin (blue; CNPase) ensheathed a majority of spiral ganglion neurons(red;Tuj1)


Credit: Hainan Lang,MD, PhD; Medical University of South Carolina; Department of Pathology and Laboratory Medici

Description:  N/A


Credit: Liana Sargsyan and Hongzhe Li, PhD at VA Loma Linda Healthcare System

Description: The mid turn of the cochlea of a 7-week-old C57BL/6 mouse, after a single dose of systemic gentamicin treatment followed by furosemide injection, with immunofluorescent labeling. Antibodies: mouse(IgG1) anti-CtBP2 and goat anti-mouse (IgG1)-AF 568 were used to identify nuclei and ribbon synapses(red). Phalloidin 488 was used to identify outer hair cells (green) in the basal turn of the cochlea.


Credit: Liana Sargsyan and Hongzhe Li, PhD at VA Loma Linda Healthcare System

Description: The basal turn of the cochlea of a 6-week-old mouse in C57BL/6 background, with immunofluorescent labeling. Antibodies: mouse (IgG1) anti-CtBP2 and goat anti-mouse (IgG1)-AF 568 were used to identify nuclei and ribbon synapses (red).Phalloidin 488 was used to identify outer hair cells (green), in the basal turn of the cochlea.


Credit: Liana Sargsyan and Hongzhe Li, PhD at VA Loma Linda Healthcare System

Description: The mid turn of the cochlea of a 6-week-old mouse lacking Duffy antigen receptor for chemokines, with immunofluorescent labeling. Antibodies: mouse (IgG1)anti-CtBP2 and goat anti-mouse (IgG1)-AF 568 were used to identify nuclei and ribbon synapses (red). Phalloidin 488 was used to identify outer hair cells (green), in the mid turn of the cochlea


Credit: Liana Sargsyan and Hongzhe Li, PhD at VA Loma Linda Healthcare System

Description: The mid turn of the cochlea of a 7-week-old C57BL/6 mouse, after a single dose of gentamicin injection,with immunofluorescent labeling. Antibodies: mouse (IgG1) anti-CtBP2 and goat anti-mouse (IgG1)-AF568 were used to identify nuclei and ribbon synapses (red). Anti-neurofilament and AF 488 goat anti-rabbit IgG (H+L) used to identify auditory nerve fibers (green)


Credit: Liana Sargsyan and Hongzhe Li, PhD at VA Loma Linda Healthcare System

Description: The apex of the cochlea of a 6-week-old C57BL/6 mouse, after intratympanic lipopolysaccharide injection, with immunofluorescent labeling. Antibodies: rabbit mAb to IBA1 and anti-rabbit IgG (H+L)were used to identify immune (Iba1+) cells (magenta), and Phalloidin 647 to identify outer hair cells(blue


Credit: Mark Rutherford

Description: Midcochlear organ of Corti (45 kHz region) from ap34 C57BL/6J male mouse. Antibodies label the inner and outer hair cells (blue, Myosin7a), spiral ganglion neurons and medial olivocochlear neurons (red,NaKATPase), and voltage-gated Na+channels (green,NaV1)


Credit: Patrick Lam, Ella Trang, Niki Gunewardene, and Andrew Wise of Bionics Institute

Description: A 3-day culture of primary spiral ganglion cells isolated from 5D neonatal Sprague Dawley rats.This image shows a common phenomenon of spiral ganglion neuron cell bodies being surrounded by glial cells that are likely to share a reciprocal relationship with the seneurons. Cells were visualized via immunocytochemistry. Labelled in green are two spiral ganglion neurons using Tuj1 as the primary antibody binding marker; in red are the glial cells using S100 beta as the primary antibody binding marker; all cell nuclei were stained with DAPI in blue.


Credit: Randy J. Kulesza, Jr. and Yusra Mansour

Description: 3D reconstructions of the nuclei of the Superior Olivary Complex of a neurotypical child (left;8-year-old female) compared to that of a child with Autism Spectrum Disorder (right;8-year-old male).The 3D models were constructed in Amira from Geimsa-stained sections.There is a significant decrease in overall nuclear volume and highly irregular contours of each of the SOC nuclei, in the ASD subject compared to the NT subject, with a preferential effect on theMSO. Key to colors: Medial Superior Olive = red, Lateral Superior Olive = blue, Superior Paraolivary Nucleus = yellow, Medial Nucleus of the Trapezoid Body = green, Ventral Nucleus of the Trapezoid Body = magenta, Lateral Nucleus of the Trapezoid Body = orange.


Credit: Randy J. Kulesza, Jr. and Yusra Mansour

Description: The figure shows a coronal section through the rat central nucleus of the inferior colliculus. Wisteria floribunda-positive Perineuronal nets (cyan) are seen to surround cell bodies and primary dendrites of select neurons. Neurons and glial cells are labelled with Neurotrace Red (magenta)

 


Credit: Shiran Wolland, Roie Cohen, & Shahar Taiber. School of Neurobiology, Biochemistry and Biophysics, George S. Wise Faculty of Life Sciences, Tel Aviv University

Description: Confocal image of the organ of Corti from P0 Atoh1-mCherry-Zo1-EGFP mouse. 


Credit: Leonardo R. Andrade of Salk Institute for Biological Studies

Description: SEM image showing a 18 days old mouse Organ of Corti with the lookup table gem


Credit: Leonardo R. Andrade of Salk Institute for Biological Studies

Description: Freeze-fracture deep etch of a guinea pig outer hair cell showing the lateral wallwhere prestin motors for cell motility are localized


Credit: Leonardo R. Andrade of Salk Institute for Biological Studies

Description: SEM of bullfrog basilar papilla with two hair cells at opposite orientation

 

To view past photo contest winners and participants, click HERE.